SYNTHESIS AND CHARACTERIZATION OF CANCRINITE-TYPE ZEOLITES MODIFIED WITH Ag AGAINST ENTEROPATHOGENIC BACTERIAS (Escherichia coli, Shigella spp and Salmonella spp)

Abstract

Gastrointestinal infections, caused by enteropathogenic bacteria such as Escherichia coli, Shigella spp and Salmonella spp, can produce acute inflammatory or dysenteric diarrhea, characterized by the presence of frequent stools with blood and mucus, accompanied by symptoms such as fever, abdominal pain and rectal tenesmus. On the other hand, cancrinite type zeolite is an open network that allows the exchange of cationic species that can have a pharmacological effect. In the present work, a cancrinite-type zeolite was synthesized under the nitrate / carbonate anionic mixture using commercial zeolite X at 80 ° C, silicon and aluminum, 24 hours of crystallization and autogenous pressure. Subsequently, this zeolite was exchanged with Ag under anaerobic conditions and under reflux. The zeolites were characterized by the techniques of infrared spectroscopy with Fourier transform (FT-IR) and X-ray diffraction (XRD). Finally, the antibacterial effect of the modified cancrinite was tested in a population of enteropathogenic bacteria belonging to the family Enterobacteriacea, with Escherichia coli, Shigella spp and Salmonella spp being chosen as samples. The tests were carried out in the presence of Mueller-Hinton broth supplemented with variable amounts (1.0-5.0) mg of the cancrinite exchanged with Ag at 37 ° C during a period of 24 h of incubation. A system with sodium-nitrated cancrinite was incubated as a positive growth control. The results indicated that this solid with Ag is able to exert a bactericidal effect against Escherichia coli and bacteriostatic against Shigella spp., And Salmonella spp.